Laboratory Research Technologist needed to perform daily operations of a research laboratory investigating the inflammatory mechanisms involved in lung disease (especially emphysema) in animal models
Position will support research projects looking at the particular role of macrophages in lung diseases, and it will also involve interactions with graduate students and other faculty members. The ultimate goal of the lab research is to develop therapies helpful in dealing with the global increase in lung disease. The lab work requires basic use of scientific methods, procedures and techniques in physiology, immunology, and simple surgery in mice, but these skills can all be learned with on the job training. Basic computer skills with Mac and/or Windows OS is essential. Previous experience is not necessary, but a strong motivation and work ethic is required. A ability to perform data analysis and troubleshoot occasional problems will enable a substantial degree of independence.
Maintain mouse colonies
Anesthetize and ventilate mice with a computer-controlled ventilator
Perform isolation of organs, tissues and cells for subsequent processing
Perform RT-qPCR and endpoint PCR,
Perform polyacrylamide and agarose gel electrophoresis
Perform light, fluorescent, and confocal microscopy including image acquisition.
Record and analyze results, organize data, perform basic computations, and develop reports and summaries of analyses;
Maintain reports of all lab experiments and data acquired
Operate basic and advanced laboratory equipment, including gas chromatograph, gas tanks, computer analyses, digital physiologic recorder,
Other responsibilities include:
Contribute to general maintenance of the laboratory, equipment and supplies;
Maintain and make minor adjustments to equipment, e.g. calibrations;
Monitor inventory levels, order materials and supplies in accordance with established policies and maintain records.
Bachelor's degree in biology, chemistry or a related field required.
Some related post-undergraduate work experience preferred.
Special Knowledge, Skills, and Abilities:
Willingness to work independently. Basic lab techniques, handling of experimental mice, surgical removal of organs for fixation and histology, cell culture, immunological assays.
Equipment to be used:
Mechanical ventilator, microscope, gas chromatograph,
Classified title: Research Technologist
Working title: Research Technologist
Role/Level/Range: ACRO 40/E/02/CC
Starting Salary Range: $14.14 - $19.44 (commensurate with experience)
Employee group: Fulltime
Employee Status: Non-Exempt
Schedule: Monday-Friday –9:00am-5:00pm 40hrs/Wk
Location: BSPH Campus – Baltimore, MD
Department name: 10001082-Environmental Health and Engineering
Personnel area (School): BSPH – Bloomberg School of Public Health
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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