The purpose of this position is to carry out the daily tasks of a Core facility laboratory. This position will set up sequencing reactions, gene expression and genotyping assays, distribute oligonucleotides and other products to customers, interact with and assist customers using self-service qPCR equipment. This position will interact with students, technicians and primary investigators as needed, including troubleshooting customer samples and experiments. This position must be able to provide exceptional customer service and maintain meticulous work standards.
Specific Duties & Responsibilities
With minimal supervision, follow protocols to carry out sequencing reactions, gene expression assays, genotyping assays, pyrosequencing, digital PCR and other experiments as necessary.
Set up sequencing reactions and run DNA Sequencer.
Set up gene expression/genotyping assays and run associated equipment.
Follow QA/QC protocols and all laboratory safety protocols.
Responsible for daily maintenance of the equipment and proper use.
Monitor laboratory supplies and order supplies when needed.
Operate the pyrosequencing and digital PCR instruments independently.
Assess data and release to customers.
Contact, interact with, and assist customers as needed.
Troubleshoot customer experiments and provide assistance as needed.
Keep track of self-service genotyping use and maintain iLab billing records.
Ensure that client requests are entered properly into database.
Ensure that samples are properly labeled and match requests.
Receive oligonucleotide delivers and orders and update database as necessary.
Receive, log and track orders for delivery to our off-site locations. Deliver oligonucleotides and other products to customers as needed.
Answer phones when needed.
Willing to cross-train in human blood processing, cell culture and single cell genomic methods.
Perform miscellaneous related duties as assigned.
Minimum Qualifications (Required)
Bachelor's degree in biology, chemistry or related field.
Some related post-undergraduate work experience preferred.
Experience in a Core facility setting.
Special Knowledge, Skills, and Abilities
Experience with the following equipment helpful, but will train as necessary.
Centrifuges, electrophoresis equipment, 3730XL DNA Analyzers,
Real-Time PCR equipment (including the QuantStudio qPCR instruments),
Digital PCR, PCR thermocycler, robotic and manual pipettors, common lab equipment (hotplate/stirrers, balances, spectrophotometer, etc.),
Macintosh and IBM computers; 95% time.
Sitting in a normal seated position for extended periods of time;
Reaching by extending hand(s) or arm(s) in any direction;
Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard; Communication skills using the spoken word;
Ability to see and hear within normal parameters;
Ability to move about workspace. Lift, carry, move supplies, computers, etc.
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Hourly Pay Rate Range: $14.14 - $19.44 /Commensurate with Experience Employee group: Full Time Schedule: Monday - Friday, 8:30a - 5:00p / 40 hrs per week Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10002886-SOM Genetic Resources Core Facility GRCF Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at [email protected]. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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