We seek a researcher 1 to contribute to testing an oncolytic virus to treat peripheral nerve sheath tumors. Mouse husbandry, tissue culture and molecular analyses. Also, this staff person will help to conduct genome wide CRISPR/Cas9 screens in vitro to define synthetic lethal interactions with loss of certain tumor suppressor gene.
Mouse husbandry (breeding, tumor injection, tumor measurements) - 40% This includes assisting in the on-going animal studies to maintain mice with human tumor xenografts, assisting in performing therapeutic studies with mice including intratumoral injection of oncolytic adenoviruses and tumor size measurements, performing mouse necropsies.
• In vitro drug testing in tissue culture - 10% This includes standard cell biology/genetics laboratory tasks such as culturing mammalian cells, maintenance of variety of cancer cell lines, protein isolation, western blotting, transfection or transduction of the human cell lines, adenovirus replication assays in cultured cells.
• Microscopy / histopathology- 10% This includes fixing, staining, and imaging of cells or tumor tissues, preparing tumor samples for IHC including sectioning, staining and imaging.
• Biochemistry and molecular biology - 20% This includes standard biochemical techniques such as DNA/RNA purification, bacterial transformation, PCR, standard molecular cloning techniques using restriction enzymes, analysis of DNA sequencing files.
• Record keeping - 20% Maintaining good laboratory notebooks and digital data files, accurately recording data, experimental designs etc.
All required qualifications must be documented on application materials.
-BA/BS or a combination of related education and work experience to equal four years; applies knowledge and skill to a recurring task or activity with emphasis typically on precision and timeliness of execution. -Laboratory experience including work with tissue culture cells and lab mice
Preferred Qualifications: -B.S. or B.A. in Biological sciences or related -Cancer biology experience -Molecular biology including PCR, western blotting, histopathology -Immunohistochemistry
Internal Number: 335497
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