University of Iowa Health Care Department of Internal Medicine, Division of Pulmonary, Critical Care and Occupational Medicine is seeking a Research Assistant to conduct experimental laboratory research and procedures in the area of immunology and pathologic viral infection. The lab studies immune responses to influenza virus using mouse models and samples obtained from human subjects. The work includes analysis of immune responses in the lung and ex vivo immunological assays. Responsibilities include mouse husbandry; conducting research activities/experiments; collecting data; and maintaining the overall function of laboratory through management of supplies, facilities and equipment.Duties to include:
KEY AREAS OF RESPONSIBILITY
Perform mouse husbandry, including breeding, screening and genotyping.
Perform research activities based upon protocols developed by other research staff or principal investigator of the research project. Contribute to the planning of experiments.
Experiments, Investigations, Evaluations:
Read and understand protocols.
Arrange and plan daily activities to prepare for research protocols.
Set up and operate research materials or equipment.
Perform procedures and experiments assuring fidelity to protocols. Specifically, experiments will include collection of lymphoid tissue and purification of leukocytes, performing basic cellular and molecular biology assays (DNA and RNA purification, PCR, Western blots, cell culture), production of the mouse-adapted virus used in the animal models, performing animal models (infection, monitoring, tissue collection), processing human samples, tissue culture and flow cytometry (staining, running samples on the cytometer).
Adhere to safety and compliance guidelines.
Collect, record and consolidate data as directed by the Principal Investigator.
Track progress of research activities.
Adhere to Quality Assurance protocols to maintain validity and integrity of research data.
As individual skills develop: troubleshoot, analyze and propose modifications to protocols; perform complex techniques per protocol; test and evaluate current procedures; develop and implement procedures for monitoring data integrity; present results at team meetings.
Collect, Analyze and Summarize Data:
Collect and track, perform data entry for reports and maintain records of methods used.
Prepare materials for reports.
As skills develop, analyze data.
May present results/finding at team meetings.
Administrative and Project Management:
Assist with IRB and IACUC protocol submissions.
Track various indicators of project progress and summarize in reports to the research team.
Assist in documenting compliance with safety and regulatory guidelines.
Facilities and Equipment Management:
Assist in the preparation, calibration, and maintenance of laboratory and facility equipment.
Assist with procurement and organization of supplies and materials.
Regular salaried position located in Iowa City, Iowa
Fringe benefit package including paid vacation; sick leave; health, dental, life and disability insurance options; and generous employer contributions into retirement plans
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The University of Iowa offers a generous benefits package, including paid vacation and paid sick leave. Complete information regarding the full benefits package may be viewed at: .
A Bachelor's degree in Biology is required.
Excellent written and verbal communication skills, and basic computer literacy (Microsoft Word, Excel, PowerPoint, Outlook, LabArchives, Quartzy), are required.
Experience with the following basic laboratory techniques is required: PCR, gel electrophoresis, DNA purification and sequencing, cell staining and imaging via microscopy, aseptic culture techniques, ELISA, immunoprecipitation and Western blotting
Experience with viral or bacterial culture is required.
Experience with small animal handling and dissection is required.
Application Process: In order to be considered, applicants must upload a resume, cover letter and 3 references that clearly address how they meet the listed required and desired qualifications of this position.
Job openings are posted for a minimum of 14 calendar days.
Successful candidates will be subject to a criminal background check.
The University of Iowa is an equal opportunity/affirmative action employer. All qualified applicants are encouraged to apply and will receive consideration for employment free from discrimination on the basis of race, creed, color, national origin, age, sex, pregnancy, sexual orientation, gender identity, genetic information, religion, associational preference, status as a qualified individual with a disability, or status as a protected veteran.
Internal Number: 20001054_1578412752
About University of Iowa
With over 31,000 students, the University of Iowa is one of the nation's top public research universities, a member of the Big Ten conference and the Association of American Universities. The University of Iowa is composed of eleven colleges and is known around the world for its balanced commitment to the arts, sciences, and humanities. It is home to one of the nation's largest academic medical centers and the pioneering Iowa Writers' Workshop. The University of Iowa promotes work/life integration and is located in the casual yet cosmopolitan environment of Iowa City, widely recognized as one of the country's most livable communities.