Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutchs pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nations first cancer prevention research program, as well as the clinical coordinating center of the Womens Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
This research opportunity is with the McElrath Lab within the Vaccine & Infectious Disease Division. The technician will work under the direction of a Staff Scientist and use discretion and judgment in performance of duties in support of human mucosal immunology studies relevant to HIV-1 prevention. In particular, they will perform and assist in the development of mucosal explant models for evaluating the functionality, levels and distribution of HIV-1 specific antibodies. They will also be responsible for processing various mucosal specimens from study participants, performing downstream assays, collecting and analyzing the data.
The incumbent will work closely with other laboratory staff conducting mucosal studies. They will interpret and report on experimental results and assist with data presentation for conferences and manuscripts. They may work with the clinical staff from the Fred Hutch HIV vaccine Trials Unit, University of Washington Medical Center (UWMC) and other hospitals to coordinate specimen transfer and/or personally pick up specimens. They will assist in the development and maintenance of laboratory quality control (QC) methods and records, which may require adherence to GCLP (Good Clinical Lab Practice) standards
***A cover letter is required for consideration for this position***
Coordinate specimen collection and transport from UWMC and other clinics
Coordinate sample shipping/receiving with collaborating laboratories
Process cervicovaginal, rectal and other mucosal tissue specimens
Process cervicovaginal secretions, rectal secretion and seminal plasma
Be available to process additional mucosal specimens as needed (e.g. cervical cytobrushes)
Perform ex vivo infectivity assays on mucosal tissues
Perform luminescence reporter assays to measure HIV infection
Prepare single cell suspension from mucosal specimens, such as cytobrushes and mucosal tissues
May prepare and cryopreserve PBMC
Perform microdissection of mucosal tissue to separate epithelium from underlying stroma
Perform and assist in developing immunohistochemistry assays on mucosal tissues
May perform confocal microscopy, including receptor and virus co-localization in mucosal tissue
Perform and assist in developing flow cytometric assays (e.g. both phenotyping and functional assays)
Perform and assist in developing ELISAs and related assays to measure mucosal analytes (e.g. mucosal antibodies)
Perform and/or help optimize new assays and specimen processing methods as needed
May perform and help optimize qPCR and/or digital droplet PCR assay (e.g. for detecting HIV-1 infection)
May prepare stocks of HIV isolates by transient transfection
Maintain records for experiments and quality control for the laboratory
Operate and carry out proper maintenance and QC of laboratory equipment
Prepare and maintain reagents, stocks, and cultures necessary to perform work
Order and maintain laboratory supplies
Other duties as assigned
A bachelors degree in a biological science and some bench research experience or the equivalent combination of education and experience. Experience with cellular immunologic assays, tissue culture, micro-dissection, molecular biology and microscopy is desirable but not essential. Motivation and adaptability are essential, as is a keen interest in the HIV vaccine and prevention fields. Must be willing to work with biohazardous/infectious agents (e.g., blood-borne pathogens, including HIV-1), including human tissues under carefully controlled conditions. Strong organizational and communication skills are necessary. Must be flexible and willing to work some evenings and weekends.
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At Fred Hutchinson Cancer Research Center, teams of world-renowned scientists and humanitarians work together to prevent, diagnose and treat cancer, HIV/AIDS and other diseases. Researchers are discovering new ways to detect cancers earlier, improve treatments, and learn how to prevent cancers from growing. Although Fred Hutchinson opened its doors in 1975, its history began about 20 years before that. In 1962 Fred Hutchinson envisioned a center devoted to studying cancer, a disease that took his brother’s life. Today Fred Hutchinson is contributing to the next waves of breakthrough treatments and prevention strategies. Fred Hutchinson collaborates with the Seattle Cancer Alliance, the National Cancer Institute, and the University of Washington.