Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status.
Base pay is commensurate with experience.
The Cryo-Electron Microscopy Specialist will work with existing cryo-EM staff and other center EM staff to support the operation of the Titan Krios G3 Cryo-EM equipped with a Direct Electron Detector for single particle and tomography studies. This position will work with other EM staff under the supervision of the Director in operation of the electron microscopy component of the Center for Cellular Imaging (http://wucci.wustl.edu/). The Cryo-EM Specialist will also have the opportunity to work on correlative microscopy technology development projects with the other cryo-EM staff and the Center Director.
Provides critically complex research and experimentation for department/s allowing the advancement of scientific analysis and discovery. Serves as a subject matter expert offering support, training and guidance relative to the discipline/area. May participate and or present on the research developed, or the established work of those within the department.
PRIMARY DUTIES AND RESPONSIBILITIES:
User Training - Train users in sample preparation and imaging techniques for single-particle cryo-EM.
Introduce and orient new users on the safe operation of the Titan Krios cryo-EM platform.
Provide assistance and troubleshooting on cryo-EM experiments.
Provide advanced level instruction on an individual level with undergraduate students, graduate students, post-docs, and collaborators on the appropriate methods to vitrify samples (both for single particle and tomography).
Develop reference tools for lab staff including handouts.
Cryo-EM Service - Prepare samples for single particle cryo-EM and collect and reconstruct images for University researchers to help to prepare data for publication and presentations.
Prepare biological samples for cryo-EM (purified proteins and cells) using plunge freezing techniques.
Data collection and evaluation of data for in-house and external users carrying out structural biology-focused projects.
Evaluate quality of data obtained by users.
Interface and collaborate with other EM staff in the Center to facilitate preliminary screening of samples via negative staining techniques.
Basic 3D image processing of single particle and/or tomography datasets.
Interface with Scientific Computing team, who will assist with computational needs for optimal data storage and data processing.
Prepare data for publication; grant applications, posters and other presentations using digital facilities.
Prepare publication quality micrographs or images/movies for presentations.
Assist in preparation of manuscripts, grants, progress reports and annual protocols.
Maintain lab notebook, including experimental design, time, methods, materials and results.
Cryo-EM Equipment Maintenance - Maintenance of the cryo-EM and sample preparation equipment.
Liaise with manufacturer (FEI/Thermo Fishr) with regard to instrument preventative maintenance.
Maintain ancillary, cryo-EM sample preparation equipment such as Vitrobot, glow discharger etc.
Load samples and fine-tune microscope prior to single particle or tomography data collection runs.
Maintain records of equipment usage and report maintenance issues of equipment.
Ensure assigned areas, and various pieces of equipment are clean and prepared.
Ensure Center is in compliance with EH&S protocols and procedures.
Master’s degree in a related field with 7 years of experience in a laboratory setting or equivalent combination of relevant education and work experience equaling at least 10 years.
This position is full-time and works approximately 40 hours per week.
Department Name/Job Location:
This position at the Center for Cellular Imaging is affiliated with the Departments of Neuroscience and Cell Biology & Physiology. This position is for the Medical School Campus.
At least 2-5 years of cryo-EM experience, with expertise in sample freezing, sample handling, microscope use, and data evaluation and analysis.
Firm understanding of the principles of Transmission Electron Microscopy (TEM).
Demonstrated skill and experience in the high-resolution operation of cryo-electron microscopes.
General understanding of single-particle cryo-EM workflow, experience in cryo-electron tomography, Cs correction and Phase contrast systems would be added benefits.
Theoretical and practical knowledge of biology or related scientific field and of tissue structure on an anatomical, cellular, and molecular level.
Ability to contribute to experimental design and assist with the analysis and interpretation of results.
Proven organizational and time management skills to successfully set priorities, meet established deadlines and recognize new problems with constantly changing priorities and frequent interruptions.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment. Ability to read, comprehend, and discuss research materials. Proven ability to write, edit and proofread research results. Skill at explaining difficult concepts and training users and students.
Ability to diplomatically and professionally interact with all levels of University faculty and staff and external contacts.
Proven ability to perform minor maintenance and alignment of electron microscopes.
Experience in single-particle cryo-EM reconstruction using RELION.
Experience in cryo-electron tomography reconstructions using IMOD and/or Tomo3D.
Experience with macromolecular model building in cryo-EM density maps.
Experience in working in a Linux/UNIX environment and with cluster computing.
Please attach a copy of your most current signed performance evaluation (completed within the last 18 months) to your online account. If you have not received a performance evaluation, you may provide two current signed letters of recommendation (written within the last 18 months), preferably to include one letter from either a current or recent former supervisor. To attach these documents, go to: My Career Tools, Add Attachment, Attachment Type – Performance Reviews or Letters of Recommendation.
All external candidates receiving an offer for employment will be required to submit to pre-employment screening for this position. Current employees applying for a new position within the university may be subject to this requirement. The screenings will include a criminal background check and, as applicable for the position, other background checks, drug screen, employment and education or licensure/certification verification, physical examination, certain vaccinations and/or governmental registry checks. All offers are contingent upon successful completion of required screening.
Washington University in St. Louis, a medium-sized, independent university, is dedicated to challenging its faculty and students alike to seek new knowledge and greater understanding of an ever-changing, multicultural world. The University offers more than 90 programs and almost 1,500 courses leading to bachelor's, master's and doctoral degrees in a broad spectrum of traditional and interdisciplinary fields, with additional opportunities for minor concentrations and individualized programs. The faculty is composed of scholars, scientists, artists and members of the learned professions. They serve society by teaching; by adding to the store of human art, creativity, understanding, and wisdom; and by providing direct services, such as health care.