Job Requisition Number: 26069. The Department of Molecular & Cell Biology (MCB) is home to Undergraduate and Graduate academic programs as well as Faculty Research performed by faculty organized in five divisions:
•Biochemistry, Biophysics and Structural Biology •Cell and Developmental Biology •Genetics, Genomics and Development •Immunology and Pathogenesis •Neurobiology
As a department, we are committed to supporting all our members and increasing the representation of historically under-represented groups in our community.
Using sterile technique in a vertical laminar flow hood, change medium and split cell cultures (up to 100 different lines per week), both as maintenance and as needed for experiments by researchers. Assess the health of cultures visually and by trypan blue exclusion. Remove samples sterilely and count viable cells using a hemacytometer or automated cell counter. Freeze and thaw cultures as requested or upon independent observation of health and age of cultures. Maintain records of all work. Create and maintain databases using FileMaker Pro. Set up plates, dishes or flasks of cells and perform cell culture experiments. Independently decide the best concentration of cells or decide which cells are best for the type of experiment and then set up experiments. Advise authors of papers and theses regarding 'materials and methods' sections. Adapt and/or modify procedures for use in this facility. Research characteristics and special growth requirements of new additions to the cell bank. Prepare and analyze samples for mycoplasma and short tandem repeat analysis. Adapt current cell lines to suspension cultures upon clients request, involves troubleshooting. Research and propose new services and cultures. Independently modifies protocols.
Serve as senior staff assigned to the Tissue Culture, Media, Fly Food and On-Call Facilities. Organize and manage the use and maintenance of rooms comprising the Tissue Culture Facility. Manage and supervise use of all facility equipment. Provide public service in the form of cultures and curatorial-technical advice to researchers from over 40 different labs. Answer email and phone calls from researchers ordering cells and asking advice and organize the weekly cell splitting schedule. Manage online ordering system, store client chart strings and set up new client accounts. Maintain and keep inventory of frozen stocks of cell lines. Update chemical inventory. Inventory, order and stock supplies. Responsible for all advertising for the facility, digital and print. Act as liaison to industry clients. Contact vendors for quotes and RFPs. Plan and execute Open House events.
Instruct undergraduate and graduate students and postdoctoral fellows in proper cell culture technique, appropriate growth media and reagents. Instruct clients in fee-for-training course. Assign work to the student employees. Make and enforce work rules for the facility. Supervise the work habits of all who use the facility. Participate in interview process for all new employees. Solely responsible for undergraduate training.•B.A./B.S. in biological sciences, or equivalent combination of education and experience. •Basic Chemistry and/or Biology courses plus lab course work •Experience in sterile technique •Demonstrated experience maintaining mammalian cell cultures •Computer proficiency •Experience in lab management/maintenance •Willingness to learn new techniques/skills to improve efficiency/integrity of work •Demonstrate experience in working with a variety of laboratory instrumentation, including ability to troubleshoot equipment whenever problems arise and resolve problems quickly so that work can be done in the facility with minimal disruption •Must be able to follow oral and written instructions and perform mathematical calculations pertaining to solution preparation and cell splitting ratios •Must be able to work well without direct supervision after training period •Knowledge of laboratory safety techniques and procedures •Ability to adhere to EH&S Safety Policies and Procedures •Must have excellent record keeping abilities. •Strong organizational and problem solving skills, and the ability to analyze and interpret data •Ability to work effectively, both independently and as a team member, with students and staff, including individuals from a wide variety of cultures and backgrounds •Ability to function well under pressure and achieve results •Must be willing to work more than 8 hours on Mondays and Fridays, holidays, and occasionally a few hours on Saturday, when necessary, for an average of 40 hours per week
•B.A./B.S. in biological sciences, or equivalent combination of education and experience. •Basic Chemistry and/or Biology courses plus lab course work •Experience in sterile technique and mammalian cell culture
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