Department name: 10002808-SOM DOM Gastroenterology
Work Schedule: Mon-Fri 8-5
Location: 04-MD:School of Medicine Campus Personnel area: School of Medicine
The Division of Gastroenterology and Hepatology at the Johns Hopkins University School of Medicine is seeking a Research Specialist whose position will be funded by the National Institutes of Health. The Research Specialist will individually and collectively contribute to current research projects related to human intestinal epithelial stem cell physiology and pathophysiology as well as support laboratory other members, equipment and inventory. He/she will support other lab members with primary human stem cell culture models. The Research Specialist will regularly work in collaboration with individuals in the lab and with collaborators from other institutions.
Specific duties and responsibilities:
Performs laboratory tests which include cell culture of primary human cells, immunostaining, SDS-PAGE and Western blot, qRT-PCR, flow cytometry, and enzyme-linked immunosorbent assay (ELISA). Operates requisite laboratory equipment and instruments; records data; maintains and makes minor adjustments to equipment. Uses universal safety precautions to protect self and co-workers from biohazardous materials. Complies with biohazard safety standards through proper handling of potentially hazardous chemical and biological agents in the workplace.
Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Uses sterile techniques to avoid contaminating lab experiments. Prepares, cleans, sterilizes, and maintains laboratory equipment, glassware, and instruments used in research experiments.
Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Bachelor's degree in biology, chemistry, or related field plus minimum of one year of laboratory experience performing relevant techniques. Master's degree, with related graduate research may substitute for experience.
Related Master's degree and experience in protein biochemistry, microbiology, immunology, and cell culture are desired
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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